Variable region differences affect antibody binding to immobilized but not soluble antigen

We have examined the antigen binding characteristics of two chimeric IgG, antibodies that differ only inthe heavy chain variable region. Antibodies lOB and Bll were expressed from two different anti-(Tyr, Glu)Ala-Lys murine VH genes joined to human IgG, constant region genes in a murine anti-(Tyr, Glu)-AlaLysheavy chain loss variant hybridoma...